hcc cell lines mhcc97h  (Servicebio Inc)

Journal: Chinese Medical Journal

Article Title: USP25 promotes hepatocellular carcinoma progression by interacting with TRIM21 via the Wnt/β-catenin signaling pathway

doi: 10.1097/CM9.0000000000002714

Figure Lengend Snippet: USP25 is highly expressed in HCC and is correlated with poor prognosis. (A) The expression of USP25 in pan-carcinoma as determined with the TIMER database. (B) The expression of USP25 mRNA was analyzed in HCC tissues from TCGA and ICGC databases. (C) The protein expression levels of USP25 in a normal liver cell line and six HCC cell lines. (D) The expression of USP25 in different HCC cell lines in the CCLE database. (E) Kaplan–Meier survival analysis of survival in HCC patients grouped according to USP25 expression ( n = 370). (F) The promoter methylation level of USP25 in different grades of HCC according to the analysis of patient samples. Grade 1: well differentiated; Grade 2: moderately-differentiated; Grade 3: moderately and poorly differentiated; Grade 4: poorly differentiated. (G) The expression of USP25 in HCC patients based on nodal metastasis status. * P <0.05; † P <0.001; ‡ P <0.01, § P <0.0001. ACC: Adrenocortieal carcinoma; BLCA: Bladder urothelial carcinoma; BRCA:Breast invasive carcinoma; CESC: Cervical squamous cell carcinoma; CHOL: Cholangiocarcinoma; COAD: Colon adenocarcinoma; DLBC: Lymphoid neoplasm diffuse large B-cell lymphoma; ESCA: Esophageal carcinoma; GBM: Glioblastoma multiforme; HNSC: head and neck squamous cell carcinoma; KICH: Kidney chromophobe; KIRC: Kidney renal clear cell carcinoma; KIRP: Kidney renal papillary cell carcinoma; LAML: Acute myeloid leukemia; LGG: Brain lower grade glioma; LIHC: Liver hepatocellular carcinoma; LUAD: Lung adenocarcinoma; LUSC: Lung squamous cell carcinoma; MESO: Mesothelioma; OV: Ovarian serous cystadenocarcinoma; PAAD: Pancreatic adenocarcinoma; PCPG: Pheochromocytoma and paraganglioma; PRAD: Prostate adenocarcinoma; READ: Rectum adenocarcinoma; SARC: Sarcoma; SKCM: Skin cutaneous melanoma; STAD: Stomach adenocarcinoma; TGCT: Testicular germ cell tumors; THCA: Thyroid carcinoma; THYM: Thymoma; UCEC: Uterine corpus endometrial carcinoma; UCS: Uterine carcinosarcoma; UVM: Uveal melanoma; HCC: Hepatocellular carcinoma; ICGC: International Cancer Genome Consortium; TIMER: Tumor Immune Estimation Resource; USP25: Ubiquitin-specific peptidase 25; TCGA: The Cancer Genome Atlas; CCLE: Cancer Cell Line Encyclopedia; HR: Hazard ratio; L-O2: Normal hepatocyte cell line; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; N0, 1: Node0,1.

Article Snippet: Six human HCC cell lines (HepG2, HuH7, SMMC7721, Bel7402, MHCC97H, and PLC/PRF/5) and a normal hepatocyte cell line (L-O2) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Methylation, Ubiquitin Proteomics

Journal: Chinese Medical Journal

Article Title: USP25 promotes hepatocellular carcinoma progression by interacting with TRIM21 via the Wnt/β-catenin signaling pathway

doi: 10.1097/CM9.0000000000002714

Figure Lengend Snippet: USP25 promotes HCC cell proliferation in vitro. (A–C) HepG2 and MHCC97H cell lines with USP25 overexpression or knockdown were successfully established, as verified by analysis of protein and mRNA levels and fluorescence microscopy (Original magnification × 100). (D–F) Colony formation and CCK-8 assays were performed to detect cell proliferation. (G–J) DNA synthesis was measured by EdU IF staining (Original magnification × 200). * P <0.05; † P <0.001; ‡ P <0.01.CCK-8: Cell counting kit-8; HCC: Hepatocellular carcinoma; IF: Immunofluorescence; USP25: Ubiquitin-specific peptidase 25; EdU: 5-ethnyl-2-deoxyuridine; DAPI: 4′,6-Diamidino-2′-phenylindole; shUSP25: short hairpin Ubiquitin-specific peptidase 25; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: Six human HCC cell lines (HepG2, HuH7, SMMC7721, Bel7402, MHCC97H, and PLC/PRF/5) and a normal hepatocyte cell line (L-O2) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: In Vitro, Over Expression, Knockdown, Fluorescence, Microscopy, CCK-8 Assay, DNA Synthesis, Staining, Cell Counting, Immunofluorescence, Ubiquitin Proteomics

Journal: Chinese Medical Journal

Article Title: USP25 promotes hepatocellular carcinoma progression by interacting with TRIM21 via the Wnt/β-catenin signaling pathway

doi: 10.1097/CM9.0000000000002714

Figure Lengend Snippet: USP25 promotes HCC cell migration and invasion in vitro. (A,B) A Transwell invasion assay was performed to assess the invasiveness of HepG2 and MHCC97H cells (Original magnification × 100). (C,D) A transwell migration assay was performed to analyze the migratory ability of HepG2 and MHCC97H cells (Original magnification × 100). (E–H) The scratch assay results demonstrated the migratory ability of HepG2 and MHCC97H cells (Original magnification × 40). (I,J) The expression levels of a PCNA and invasion-related proteins (MMP2 and MMP9) were detected by Western blotting. * P <0.001; † P <0.01. HCC: Hepatocellular carcinoma; PCNA: Proliferation-related protein; USP25: Ubiquitin-specific peptidase 25; MMP2: Matrix metallopeptidase 2; MMP9: Matrix metallopeptidase 9; shUSP25: short hairpin Ubiquitin-specific peptidase 25; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: Six human HCC cell lines (HepG2, HuH7, SMMC7721, Bel7402, MHCC97H, and PLC/PRF/5) and a normal hepatocyte cell line (L-O2) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, In Vitro, Transwell Invasion Assay, Transwell Migration Assay, Wound Healing Assay, Expressing, Western Blot, Ubiquitin Proteomics

Journal: Chinese Medical Journal

Article Title: USP25 promotes hepatocellular carcinoma progression by interacting with TRIM21 via the Wnt/β-catenin signaling pathway

doi: 10.1097/CM9.0000000000002714

Figure Lengend Snippet: USP25 promotes HCC growth by activating the Wnt/β-catenin signaling pathway. (A) The expression of β-catenin was observed by IF staining (Original magnification × 200). (B) The mRNA expression of components in the Wnt/β-catenin signaling pathway was measured by qRT-PCR. (C) The expression of β-catenin was observed by Western blotting of nuclear-cytoplasmic fractionation. (D, E) Western blotting was used to detect the protein expression levels of Wnt/β-catenin pathway components and downstream factors. (F, G) Western blotting analysis after XAV939 treatment of HepG2 and MHCC97H cells. * P <0.001; † P <0.01; ‡ P <0.05, shUSP25 vs . Control; § P <0.05, shUSP25+XAV939 vs . Control. HCC: Hepatocellular carcinoma; IF: Immunofluorescence; qRT-PCR: Quantitative real-time polymerase chain reactio; USP25: Ubiquitin-specific peptidase 25.DAPI: 4′,6-Diamidino-2′-phenylindole; shUSP25: Short hairpin Ubiquitin-specific peptidase 25; PCNA: Proliferating cell nuclear antigen GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GSK3β: Glycogen synthase kinase 3 beta.

Article Snippet: Six human HCC cell lines (HepG2, HuH7, SMMC7721, Bel7402, MHCC97H, and PLC/PRF/5) and a normal hepatocyte cell line (L-O2) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Staining, Quantitative RT-PCR, Western Blot, Fractionation, Control, Immunofluorescence, Ubiquitin Proteomics

Journal: International Journal of Preventive Medicine

Article Title: Effect of Zebularine on Apoptotic Pathways in Hepatocellular Carcinoma Cell Lines

doi: 10.4103/ijpvm.ijpvm_191_21

Figure Lengend Snippet: The effect of zebularine (0, 10, 25, 50, 75, and 100 μM) on the viability of HCC cell lines, HCCLM3, MHCC97H, and MHCC97L. The HCC cell lines were treated without and with different doses of zebularine for 24 and 48 h, and the cell viability was investigated by MTT assay. Asterisks (*) indicate significant differences between treated and untreated cells

Article Snippet: HCC cell lines (HCCLM3, MHCC97H, and MHCC97L) were obtained from the National Cell Bank of Iran-Pasteur Institute.

Techniques: MTT Assay

Journal: Oncology Letters

Article Title: Myricetin inhibits migration and invasion of hepatocellular carcinoma MHCC97H cell line by inhibiting the EMT process

doi: 10.3892/ol.2019.10998

Figure Lengend Snippet: Effects of myricetin on activity of MHCC97H cells when treated with 0, 10, 20, 30, 40, 50, 100 and 200 µM of myricetin for 48 h. The percentage of cell viability was directly proportional to the optical density value. Compared with the control group (0 µM), the suitable concentration of myricetin for MHCC97H cells was 10–100 µM. **P<0.01.

Article Snippet: Human HCC MHCC97H cell lines (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) were cultured in high-glucose DMEM containing 10% FBS and maintained at 37°C in a humidified atmosphere containing 5% CO 2.

Techniques: Activity Assay, Control, Concentration Assay

Journal: Oncology Letters

Article Title: Myricetin inhibits migration and invasion of hepatocellular carcinoma MHCC97H cell line by inhibiting the EMT process

doi: 10.3892/ol.2019.10998

Figure Lengend Snippet: Effects of myricetin on migration and invasion of MHCC97H cells. (A) In order to investigate the migration ability of MHCC97H cells, the cells were treated with 0, 25, 50 and 100 µM of myricetin for 24 and 48 h. White lines represent the wound edge. (B) Representative images and (C) graphs showing the migration and invasion of MHCC97H cells observed after 24 and 48 h, respectively. Six visual fields (magnification, ×100) in each well were randomly counted and two wells were used for each group. **P<0.01

Article Snippet: Human HCC MHCC97H cell lines (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) were cultured in high-glucose DMEM containing 10% FBS and maintained at 37°C in a humidified atmosphere containing 5% CO 2.

Techniques: Migration

Journal: Oncology Letters

Article Title: Myricetin inhibits migration and invasion of hepatocellular carcinoma MHCC97H cell line by inhibiting the EMT process

doi: 10.3892/ol.2019.10998

Figure Lengend Snippet: Effects of myricetin on protein expression. (A) The relative mRNA expression level of E-cadherin, N-cadherin and vimentin in MHCC97H cells treated with 0, 25, 50 and 100 µM of myricetin. (B) Expression of E-cadherin, N-cadherin and vimentin in MHCC97H cells treated with 0, 25, 50 and 100 µM of myricetin with GAPDH used as a reference. (C) Relative expression level of E-cadherin, N-cadherin and vimentin in MHCC97H cells treated with 0, 25, 50 and 100 µM of myricetin. *P<0.05, **P<0.01, compared with 0 µM myricetin.

Article Snippet: Human HCC MHCC97H cell lines (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) were cultured in high-glucose DMEM containing 10% FBS and maintained at 37°C in a humidified atmosphere containing 5% CO 2.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Myricetin inhibits migration and invasion of hepatocellular carcinoma MHCC97H cell line by inhibiting the EMT process

doi: 10.3892/ol.2019.10998

Figure Lengend Snippet: Effects of myricetin on actin. The filopodia and lamellipodia at the cell edge and the fibers in MHCC97H cells treated with myricetin (0, 25, 50 and 100 µM) for 48 h. F-actin, filamentous actin.

Article Snippet: Human HCC MHCC97H cell lines (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) were cultured in high-glucose DMEM containing 10% FBS and maintained at 37°C in a humidified atmosphere containing 5% CO 2.

Techniques: